Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions

Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions

Biology Direct

10.1186/1745-6150-8-12

gold

http://dx.doi.org/10.1186/1745-6150-8-12

Arsen O Batagov, Igor V Kurochkin

07 June 2013

Batagov and Kurochkin: Exosomes secreted by human cells transport largely mRNA fragments that are enriched in the 3′-untranslated regions. Biology Direct 2013 8:12.

Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their corresponding probes is detectable on the expression microarrays. Exosomal mRNA fragmentation is characterized with a specific structural pattern. The closer to the 3′-end of the transcript the fragments are localized, the larger fraction among the secreted RNAs they constitute. Since the 3′-ends of transcripts contain elements conferring subcellular localization of mRNA and are rich in miRNA-binding sites, exosomal RNA may act as competing RNA to regulate stability, localization and translation activity of mRNAs in recipient cells.

http://creativecommons.org/licenses/by/3.0/

https://astaroar.ripplewerkz.co/communities-collections/articles/13024

1745-6150

Bioinformatics Institute