Patthara Kongsuphol, Gary C.F. Lee, Sunil K. Arya, Su Yin Chiam, Mi Kyoung Park, Miniaturized electrophoresis electrochemical protein sensor (MEEPS) for multiplexed protein detections, Sensors and Actuators B: Chemical, Volume 244, June 2017, Pages 823-830, ISSN 0925-4005, http://dx.doi.org/10.1016/j.snb.2017.01.026.
Abstract:
We developed here a Miniaturized Electrophoresis Electrochemical Protein Sensor (MEEPS) platform that is capable of detecting specific and multiplexed signals from small volume of sample (10 μL) as well as reducing the effect of background interferences. The multiplexed capability and the reduced-effect of background interference are achieved using the electrophoresis unit of the MEEPS. The small foot-print system and sensitive signal detection are enabled via electrochemical sensors unit. Using cytokines as sample, results obtained from MEEPS reveal dynamic range of detection of representative cytokine, TNF-α, at 10 ng/mL–20 μg/mL and demonstrate multiplexed detections of four different cytokines IL-6, IFN-γ, TNF-α, and IL-8 from only 10 μL of sample.