Juturu, V.; Wu, J. C., Production of high concentration of L-lactic acid from oil palm empty fruit bunch by thermophilic B. coagulans JI12. Biotechnology and Applied Biochemistry 2018, 65 (2), 145-149.
Abstract:
Thermophilic Bacillus coagulans JI12 was used to ferment hemicellulose hydrolysate obtained by acid hydrolysis of oil palm empty fruit bunch at 50 °C and pH 6, producing 105.4 g/L of l‐lactic acid with a productivity of 9.3 g/L/H by fed‐batch fermentation under unsterilized conditions. Simultaneous saccharification and fermentation (SSF) was performed at pH 5.5 and 50 °C to convert both hemicellulose hydrolysate and cellulose–lignin complex in the presence of Cellic Ctec2 cellulases using yeast extract (20 g/L) as the nitrogen source, giving 114.0 g/L of l‐lactic acid with a productivity of 5.7 g/L/H. The SSF was also conducted by replacing yeast extract with equal amount of dry Bakers’ yeast, achieving 120.0 g/L of l‐lactic acid with a productivity of 4.3 g/L/H. To the best of our knowledge, these lactic acid titers and productivities are the highest ever reported from lignocellulose hydrolysates.
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Funding Info:
Agency for Science, Technology and Research Grant Number: 1326004117
Description:
This is the peer reviewed version of the following article: Juturu, V.; Wu, J. C., Production of high concentration of L-lactic acid from oil palm empty fruit bunch by thermophilic B. coagulans JI12. Biotechnology and Applied Biochemistry 2018, 65 (2), 145-149, which has been published in final form at https://doi.org/10.1002/bab.1567. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.