Lakshmanan, M., Mohanty, B. & Lee, D. Identifying essential genes/reactions of the rice photorespiration by in silico model-based analysis. Rice 6, 20 (2013). https://doi.org/10.1186/1939-8433-6-20
Abstract:
Background:
Photorespiration, a highly wasteful process of energy dissipation, depresses the productivity of C3 plants such as rice (Oryza sativa) under dry and hot conditions. Thus, it is highly required to understand the cellular physiology and relevant metabolic states under photorespiration using systems approaches, thereby devising strategies for improving rice production.
Findings:
In silico model-driven gene deletion analysis was performed on photorespiring leaf cells under ambient and stressed environmental conditions using our central metabolic network of rice cells. As a result, we identified a number of essential genes for the cell growth across various functional pathways such as photorespiratory cycle, Calvin cycle, GS-GOGAT cycle and sucrose metabolism as well as certain inter-compartmental transporters, which are mostly in good agreement with previous experiments. Synthetic lethal (SL) screening was also performed to identify the pair of non-essential genes whose simultaneous deletion become lethal, revealing the existence of more than 220 pairs of SLs on rice central metabolism.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
This research is supported by Bioprocessing Technology Institute, A*STAR