Advance chromatin extraction enhances performance and productivity of cation exchange chromatography-based capture of Immunoglobulin G monoclonal antibodies
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Advance chromatin extraction enhances performance and productivity of cation exchange chromatography-based capture of Immunoglobulin G monoclonal antibodies
Advance chromatin extraction enhances performance and productivity of cation exchange chromatography-based capture of Immunoglobulin G monoclonal antibodies
Nian R, Gagnon P. Advance chromatin extraction enhances performance and productivity of cation exchange chromatography-based capture of Immunoglobulin G monoclonal antibodies. J Chromatogr A. 2016;1453:54-61. doi:10.1016/j.chroma.2016.05.029
Abstract:
The impact of host cell-derived chromatin was investigated on the performance and productivity of cation exchange chromatography as a method for capture-purification of an IgG monoclonal antibody. Cell culture supernatant was prepared for loading by titration to pH 6.0, dilution with water to a conductivity of 4mS/cm, then microfiltration to remove solids. DNA content was reduced 99% to 30ppm, histone host cell protein content by 76% to 6300ppm, non-histone host cell protein content by 15% to 321,000ppm, and aggregates from 33% to 15%. IgG recovery was 83%. An alternative preparation was performed, adding octanoic acid, allantoin, and electropositive particles to the harvest at pH 5.3, then removing solids. DNA content was reduced to
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
This research / project is supported by the Exploit Technologies Pte Ltd, A*STAR, under its ETPL GAP-Funded (Project No. ETPL/12-R15GAP-0009)
Description:
The full paper is available for download at the publisher's URL: https://doi.org/10.1016/j.chroma.2016.05.029