Ottenheim, C., Werner, K. A., Zimmermann, W., & Wu, J. C. (2015). Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by γ-ray induced mutagenesis. Biochemical Engineering Journal, 94, 9–14. https://doi.org/10.1016/j.bej.2014.10.020
Abstract:
Aspergillus niger DSM 26641 was exposed to 60Co γ-radiation to enhance the β-1,4-endoxylanase activity, restrict colony growth and improve robustness of pellets. The first promising mutant obtained after γ-radiation of the fungal spores at 50–2000 Gy showed a restricted colony growth and an 82% enhancement in β-1,4-endoxylanase activity. The mutant was subjected to a second round of γ-radiation at 1400 Gy generating a mutant with double the β-1,4-endoxylanase activity compared to the native strain. The selected final mutant, deposited as A. niger DSM 28712, showed a maximal saccharification activity of 26 U ml−1 on xylan based broth, 48 U ml−1 on lignocellulose hydrolysate and 375 U ml−1 on lignocellulose hydrolysate supplemented with yeast extract and mineral salts.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
Science and Engineering Research Council (SERC grant no. 0921590133, SERC grant no 1124004027), A*STAR Research Attachment Program (ARAP)